nox2 polyclonal antibody Search Results


cybb  (Bioss)
94
Bioss cybb
Cybb, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/NOX2+gp91phox+Polyclonal+Antibody/10__1080_slash_09712119__2022__2064865-90-20-22
Average 94 stars, based on 1 article reviews
cybb - by Bioz Stars, 2026-10
94/100 stars
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nox2  (Aviva Systems)
90
Aviva Systems nox2
Analysis of oxidative stress in membrane fraction of left ventricles. A, Quantification of <t>NOX2;</t> B, analysis of membrane lipids peroxidation by means of TBARS assay and C, representative immunoblot and total protein staining of NOX2 in sham‐operated group (Sham) and non‐infarcted (HFni) tissue of failing hearts. Data are presented as mean ± SEM; n = 6‐9 per group; * P < 0.05 vs Sham
Nox2, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/NOX2+Polyclonal+Antibody+(OABB00620)/pmc06714220-42-55-57
Average 90 stars, based on 1 article reviews
nox2 - by Bioz Stars, 2026-10
90/100 stars
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nox2  (Elabscience Biotechnology)
96
Elabscience Biotechnology nox2
Analysis of oxidative stress in membrane fraction of left ventricles. A, Quantification of <t>NOX2;</t> B, analysis of membrane lipids peroxidation by means of TBARS assay and C, representative immunoblot and total protein staining of NOX2 in sham‐operated group (Sham) and non‐infarcted (HFni) tissue of failing hearts. Data are presented as mean ± SEM; n = 6‐9 per group; * P < 0.05 vs Sham
Nox2, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/NOX2-gp91phox+Polyclonal+Antibody/pm40643498-112-3-12
Average 96 stars, based on 1 article reviews
nox2 - by Bioz Stars, 2026-10
96/100 stars
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alexa fluor 647 conjugated anti mouse nox2 gp91 phox  (Bioss)
93
Bioss alexa fluor 647 conjugated anti mouse nox2 gp91 phox
IMGs pre-treated with GSK2795039 (10 or 20 µM) or MCC950 (0.5 µM) for 1h prior to stimulation with LPS/nigericin (L/N; 10 µM; 30 min) in a 12-well plate. GSK2795039 (10 µM) and MCC950 (0.01 µM) were used as drug controls. L/N increased <t>NOX2</t> mean florescent intensity (MFI; A; ****p<0.0001), NOX2 activity and ROS (B,C; **p<0.01), nitrite and TNF⍺ (D,E; ****p<0.0001) compared to controls. GSK2795039 reduced NOX2 MFI (A; ++++ p<0.0001), NOX activity (B; ++ p<0.01), ROS, nitrite and TNF⍺ (C-E; ++++ p<0.0001) versus L/N. MCC950 reduced superoxide activity and ROS (B, C; + p<0.05) versus L/N but had no effect on NOX2 MFI (A), nitrite (D) or TNF⍺ (E). Data are mean ± SEM (n=3-6 per group) and are a representative of three independent experiments. **p<0.01; ****p<0.0001 versus control, + p<0.05; ++ p<0.01; ++++ p<0.0001 versus L/N; One-way ANOVA with post hoc Dunnett’s multiple comparisons test versus L/N.
Alexa Fluor 647 Conjugated Anti Mouse Nox2 Gp91 Phox, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/NOX2+gp91phox+Polyclonal+Antibody%2C+ALEXA+FLUOR+647+Conjugated/bio_rxiv__2025__05__16__654460-44-102-109
Average 93 stars, based on 1 article reviews
alexa fluor 647 conjugated anti mouse nox2 gp91 phox - by Bioz Stars, 2026-10
93/100 stars
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anti nox2 gp91phox primary antibody  (Bioss)
92
Bioss anti nox2 gp91phox primary antibody
IMGs pre-treated with GSK2795039 (10 or 20 µM) or MCC950 (0.5 µM) for 1h prior to stimulation with LPS/nigericin (L/N; 10 µM; 30 min) in a 12-well plate. GSK2795039 (10 µM) and MCC950 (0.01 µM) were used as drug controls. L/N increased <t>NOX2</t> mean florescent intensity (MFI; A; ****p<0.0001), NOX2 activity and ROS (B,C; **p<0.01), nitrite and TNF⍺ (D,E; ****p<0.0001) compared to controls. GSK2795039 reduced NOX2 MFI (A; ++++ p<0.0001), NOX activity (B; ++ p<0.01), ROS, nitrite and TNF⍺ (C-E; ++++ p<0.0001) versus L/N. MCC950 reduced superoxide activity and ROS (B, C; + p<0.05) versus L/N but had no effect on NOX2 MFI (A), nitrite (D) or TNF⍺ (E). Data are mean ± SEM (n=3-6 per group) and are a representative of three independent experiments. **p<0.01; ****p<0.0001 versus control, + p<0.05; ++ p<0.01; ++++ p<0.0001 versus L/N; One-way ANOVA with post hoc Dunnett’s multiple comparisons test versus L/N.
Anti Nox2 Gp91phox Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/NOX2+gp91phox+Polyclonal+Antibody%2C+PE+Conjugated/10__33808_slash_clinexphealthsci__1035133-81-5-12
Average 92 stars, based on 1 article reviews
anti nox2 gp91phox primary antibody - by Bioz Stars, 2026-10
92/100 stars
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rabbit polyclonal anti-nox4  (Bioworld Antibodies)
90
Bioworld Antibodies rabbit polyclonal anti-nox4
IMGs pre-treated with GSK2795039 (10 or 20 µM) or MCC950 (0.5 µM) for 1h prior to stimulation with LPS/nigericin (L/N; 10 µM; 30 min) in a 12-well plate. GSK2795039 (10 µM) and MCC950 (0.01 µM) were used as drug controls. L/N increased <t>NOX2</t> mean florescent intensity (MFI; A; ****p<0.0001), NOX2 activity and ROS (B,C; **p<0.01), nitrite and TNF⍺ (D,E; ****p<0.0001) compared to controls. GSK2795039 reduced NOX2 MFI (A; ++++ p<0.0001), NOX activity (B; ++ p<0.01), ROS, nitrite and TNF⍺ (C-E; ++++ p<0.0001) versus L/N. MCC950 reduced superoxide activity and ROS (B, C; + p<0.05) versus L/N but had no effect on NOX2 MFI (A), nitrite (D) or TNF⍺ (E). Data are mean ± SEM (n=3-6 per group) and are a representative of three independent experiments. **p<0.01; ****p<0.0001 versus control, + p<0.05; ++ p<0.01; ++++ p<0.0001 versus L/N; One-way ANOVA with post hoc Dunnett’s multiple comparisons test versus L/N.
Rabbit Polyclonal Anti Nox4, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nox2+polyclonal+antibody/rabbit+polyclonal+anti+nox2+antibody/pm28165467-84-65-69
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti-nox4 - by Bioz Stars, 2026-10
90/100 stars
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rabbit anti-human nox2/gp91phox polyclonal antibody  (Creative BioMart)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
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nox2 gp91phox polyclonal antibody, alexa fluor 555 conjugated  (Bioss)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
   Buy from Supplier
nox2 gp91phox polyclonal antibody, abby fluor 405 conjugated  (Bioss)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
   Buy from Supplier
nox2 gp91phox polyclonal antibody, abby fluor 594 conjugated  (Bioss)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
   Buy from Supplier
nox2 gp91phox polyclonal antibody, hrp conjugated  (Bioss)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
   Buy from Supplier
nox2 gp91phox polyclonal antibody, apc conjugated  (Bioss)
N/A
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on
   Buy from Supplier

Image Search Results


Analysis of oxidative stress in membrane fraction of left ventricles. A, Quantification of NOX2; B, analysis of membrane lipids peroxidation by means of TBARS assay and C, representative immunoblot and total protein staining of NOX2 in sham‐operated group (Sham) and non‐infarcted (HFni) tissue of failing hearts. Data are presented as mean ± SEM; n = 6‐9 per group; * P < 0.05 vs Sham

Journal: Journal of Cellular and Molecular Medicine

Article Title: Different signalling in infarcted and non‐infarcted areas of rat failing hearts: A role of necroptosis and inflammation

doi: 10.1111/jcmm.14536

Figure Lengend Snippet: Analysis of oxidative stress in membrane fraction of left ventricles. A, Quantification of NOX2; B, analysis of membrane lipids peroxidation by means of TBARS assay and C, representative immunoblot and total protein staining of NOX2 in sham‐operated group (Sham) and non‐infarcted (HFni) tissue of failing hearts. Data are presented as mean ± SEM; n = 6‐9 per group; * P < 0.05 vs Sham

Article Snippet: Post‐electrophoresis, proteins were transferred onto PVDF membranes (Immobilon‐P, Merck Millipore) and incubated with primary antibodies against Bax (ab182734, Abcam), Bcl‐2 (SAB4500003, Sigma‐Aldrich), Caspase‐1 (ab179515, Abcam), Caspase‐3 (#9662, Cell Signaling Technology), Caspase‐7 (#12827, Cell Signaling Technology), Caspase‐8 (#4790, Cell Signaling Technology), GAPDH‐HRP (60004, Proteintech), IL‐1β (ab9722, Abcam), MLKL (MABC604, Merck Millipore), Na/K‐ATPase (#3010, Cell Signaling Technology), NOX2 (OABB00620, Aviva Systems Biology), Cleaved PARP‐1 p25 (ab32064, Abcam), RIP1 (#3493, Cell Signaling Technology; 610459, BD Bioscience), RIP3 (ARP32835_P050, Aviva Systems Biology), pSer229‐RIP3 (ab195117, Abcam) and TNF (NBP2‐45333, Novus Biologicals).

Techniques: Membrane, TBARS Assay, Western Blot, Staining

IMGs pre-treated with GSK2795039 (10 or 20 µM) or MCC950 (0.5 µM) for 1h prior to stimulation with LPS/nigericin (L/N; 10 µM; 30 min) in a 12-well plate. GSK2795039 (10 µM) and MCC950 (0.01 µM) were used as drug controls. L/N increased NOX2 mean florescent intensity (MFI; A; ****p<0.0001), NOX2 activity and ROS (B,C; **p<0.01), nitrite and TNF⍺ (D,E; ****p<0.0001) compared to controls. GSK2795039 reduced NOX2 MFI (A; ++++ p<0.0001), NOX activity (B; ++ p<0.01), ROS, nitrite and TNF⍺ (C-E; ++++ p<0.0001) versus L/N. MCC950 reduced superoxide activity and ROS (B, C; + p<0.05) versus L/N but had no effect on NOX2 MFI (A), nitrite (D) or TNF⍺ (E). Data are mean ± SEM (n=3-6 per group) and are a representative of three independent experiments. **p<0.01; ****p<0.0001 versus control, + p<0.05; ++ p<0.01; ++++ p<0.0001 versus L/N; One-way ANOVA with post hoc Dunnett’s multiple comparisons test versus L/N.

Journal: bioRxiv

Article Title: The NOX2-ROS-NLRP3 inflammasome axis in traumatic brain injury

doi: 10.1101/2025.05.16.654460

Figure Lengend Snippet: IMGs pre-treated with GSK2795039 (10 or 20 µM) or MCC950 (0.5 µM) for 1h prior to stimulation with LPS/nigericin (L/N; 10 µM; 30 min) in a 12-well plate. GSK2795039 (10 µM) and MCC950 (0.01 µM) were used as drug controls. L/N increased NOX2 mean florescent intensity (MFI; A; ****p<0.0001), NOX2 activity and ROS (B,C; **p<0.01), nitrite and TNF⍺ (D,E; ****p<0.0001) compared to controls. GSK2795039 reduced NOX2 MFI (A; ++++ p<0.0001), NOX activity (B; ++ p<0.01), ROS, nitrite and TNF⍺ (C-E; ++++ p<0.0001) versus L/N. MCC950 reduced superoxide activity and ROS (B, C; + p<0.05) versus L/N but had no effect on NOX2 MFI (A), nitrite (D) or TNF⍺ (E). Data are mean ± SEM (n=3-6 per group) and are a representative of three independent experiments. **p<0.01; ****p<0.0001 versus control, + p<0.05; ++ p<0.01; ++++ p<0.0001 versus L/N; One-way ANOVA with post hoc Dunnett’s multiple comparisons test versus L/N.

Article Snippet: For flow cytometry, fluorophore conjugated primary antibodies were purchased as indicated: Brilliant violet-785 conjugated anti-mouse CD45 (BioLegend; Cat# 103149, RRID:AB_2564590), APC-eFluror-780 conjugated anti-mouse CD11b (Thermo Fisher Scientific; Cat# 47-0112-82, RRID:AB_1603193), Brilliant Violet-421 conjugated anti-mouse Ly6G (BioLegend; Cat# 127627, RRID:AB_10897944), Brilliant Violet–605 conjugated anti-mouse Ly6C (BD Biosciences; Cat# 563011, RRID:AB_2737949), Pe-Dazzle-594 conjugated anti-mouse CD3 (BioLegend; Cat# 100245, RRID:AB_2565882), PE-Cynanine5 conjugated anti-mouse CD4 (Thermo Fisher Scientific; Cat# 15-0041-82, RRID:AB_468695), Alexa-Fluor-700 conjugated anti-mouse CD8 (Thermo Fisher Scientific; Cat# 56-0081-82, RRID:AB_494005), CD121a (IL-1RI) Rat anti-mouse, PE, clone:35F5 (BD Biosciences; Cat # 557489, RRID: AB_396727), PerCP-eFluor 710 conjugated anti-mouse IL-1β (Thermo Fisher Scientific; Cat# 46-7114-82, RRID: AB_2573835), Alexa Fluor 647 conjugated anti-mouse NOX2/gp91 phox (Bioss; Cat# bs-3889R-A647-BSS), Dihydrorhodamine 123 (DHR123; Invitrogen; Cat # D23806),Live/Dead Fixable AQUA (Invitrogen; Cat # L34957).

Techniques: Activity Assay, Control

Adult male C57BL6/J mice were subjected to sham or CCI surgery and euthanised at 1-, 3- or 7 DPI. Tissue punches from the ipsilateral hippocampus of sham or CCI mice were collected for gene expression analysis. CCI induced increased Cyba , Cybb , Cd68 and Nlrp3 mRNA at 3 DPI (A; *p<0.05 vs sham) and there was significantly lower Cd68 mRNA at 7DPI (A; *p<0.05 vs 3 DPI). CCI increased Il1b and Tnf gene expression at 1 DPI (A; ***p<0.001 vs sham), which was lower at 3 and 7 DPI (A; *p<0.05, **p<0.01, ***p<0.001 vs 1 DPI). CCI induced increased number of infiltrating myeloid cells (CD11b + CD45 hi ) at 1- and 3 DPI as shown in the representative dot plots (B) and quantification (D; ***p<0.001; ****p<0.0001 vs sham). The number of neutrophils (Ly6G + ) peaked at 1 DPI (B,E; ****p<0.0001 vs sham), and the number of monocytes (Ly6C + ) peaked at 3 DPI (B,F; **p<0.01; ****p<0.0001 vs sham). Data are mean ± SEM (n=4-5 per group). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; by One-Way ANOVA with Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: The NOX2-ROS-NLRP3 inflammasome axis in traumatic brain injury

doi: 10.1101/2025.05.16.654460

Figure Lengend Snippet: Adult male C57BL6/J mice were subjected to sham or CCI surgery and euthanised at 1-, 3- or 7 DPI. Tissue punches from the ipsilateral hippocampus of sham or CCI mice were collected for gene expression analysis. CCI induced increased Cyba , Cybb , Cd68 and Nlrp3 mRNA at 3 DPI (A; *p<0.05 vs sham) and there was significantly lower Cd68 mRNA at 7DPI (A; *p<0.05 vs 3 DPI). CCI increased Il1b and Tnf gene expression at 1 DPI (A; ***p<0.001 vs sham), which was lower at 3 and 7 DPI (A; *p<0.05, **p<0.01, ***p<0.001 vs 1 DPI). CCI induced increased number of infiltrating myeloid cells (CD11b + CD45 hi ) at 1- and 3 DPI as shown in the representative dot plots (B) and quantification (D; ***p<0.001; ****p<0.0001 vs sham). The number of neutrophils (Ly6G + ) peaked at 1 DPI (B,E; ****p<0.0001 vs sham), and the number of monocytes (Ly6C + ) peaked at 3 DPI (B,F; **p<0.01; ****p<0.0001 vs sham). Data are mean ± SEM (n=4-5 per group). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; by One-Way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For flow cytometry, fluorophore conjugated primary antibodies were purchased as indicated: Brilliant violet-785 conjugated anti-mouse CD45 (BioLegend; Cat# 103149, RRID:AB_2564590), APC-eFluror-780 conjugated anti-mouse CD11b (Thermo Fisher Scientific; Cat# 47-0112-82, RRID:AB_1603193), Brilliant Violet-421 conjugated anti-mouse Ly6G (BioLegend; Cat# 127627, RRID:AB_10897944), Brilliant Violet–605 conjugated anti-mouse Ly6C (BD Biosciences; Cat# 563011, RRID:AB_2737949), Pe-Dazzle-594 conjugated anti-mouse CD3 (BioLegend; Cat# 100245, RRID:AB_2565882), PE-Cynanine5 conjugated anti-mouse CD4 (Thermo Fisher Scientific; Cat# 15-0041-82, RRID:AB_468695), Alexa-Fluor-700 conjugated anti-mouse CD8 (Thermo Fisher Scientific; Cat# 56-0081-82, RRID:AB_494005), CD121a (IL-1RI) Rat anti-mouse, PE, clone:35F5 (BD Biosciences; Cat # 557489, RRID: AB_396727), PerCP-eFluor 710 conjugated anti-mouse IL-1β (Thermo Fisher Scientific; Cat# 46-7114-82, RRID: AB_2573835), Alexa Fluor 647 conjugated anti-mouse NOX2/gp91 phox (Bioss; Cat# bs-3889R-A647-BSS), Dihydrorhodamine 123 (DHR123; Invitrogen; Cat # D23806),Live/Dead Fixable AQUA (Invitrogen; Cat # L34957).

Techniques: Gene Expression

Adult male C57BL6/J mice were subjected to sham or CCI surgery and euthanised at 1-, 3- or 7 DPI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers, DHR123 (ROS dye), followed by intracellular staining of NOX2 and IL-1β and analysed by flow cytometry. CCI increased #NOX2 + and #NOX2 + IL- 1β + microglia at 3- and 7 DPI as shown in the representative quadrants (A) and corresponding quantifications (B, D; *p<0.05; ***p<0.001; ****p<0.0001 vs sham). CCI increased #NOX2 + , #IL-1β + , and #NOX2 + IL-1β + neutrophils and monocytes at 1 DPI as shown in the representative quadrants (E, I) and corresponding quantifications (F-H, J-L; **p<0.01; ***p<0.001; ****p<0.0001 vs sham). Numbers reduced to sham levels at 7 DPI (F-H, J-L; *p<0.05; ***p<0.001 vs 1 and 3 DPI). Monocytic #NOX2 + peaked at 3 DPI (I, J; ****p<0.0001 vs sham). CCI induced microglial ROS production across all time-points (M, N; *p<0.05; ***p<0.001; ****p<0.0001 vs sham), which peaked at 3 DPI (N; *p<0.05 vs 7 DPI). Neutrophils and monocytes induced ROS production at 1 DPI, (M, O, P; *p<0.05 vs sham), which was reduced at 7 DPI (O, P; *p<0.05; ***p<0.001 vs 1 and 3 DPI). Monocytic ROS peaked at 3 DPI (M, P; **p<0.01 vs 1 DPI), and was reduced at 7 DPI (P; ***p<0.001 vs 3 DPI). Data are mean ± SEM (n=4-5 per group). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; by One-Way ANOVA with Tukey’s multiple comparisons test.

Journal: bioRxiv

Article Title: The NOX2-ROS-NLRP3 inflammasome axis in traumatic brain injury

doi: 10.1101/2025.05.16.654460

Figure Lengend Snippet: Adult male C57BL6/J mice were subjected to sham or CCI surgery and euthanised at 1-, 3- or 7 DPI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers, DHR123 (ROS dye), followed by intracellular staining of NOX2 and IL-1β and analysed by flow cytometry. CCI increased #NOX2 + and #NOX2 + IL- 1β + microglia at 3- and 7 DPI as shown in the representative quadrants (A) and corresponding quantifications (B, D; *p<0.05; ***p<0.001; ****p<0.0001 vs sham). CCI increased #NOX2 + , #IL-1β + , and #NOX2 + IL-1β + neutrophils and monocytes at 1 DPI as shown in the representative quadrants (E, I) and corresponding quantifications (F-H, J-L; **p<0.01; ***p<0.001; ****p<0.0001 vs sham). Numbers reduced to sham levels at 7 DPI (F-H, J-L; *p<0.05; ***p<0.001 vs 1 and 3 DPI). Monocytic #NOX2 + peaked at 3 DPI (I, J; ****p<0.0001 vs sham). CCI induced microglial ROS production across all time-points (M, N; *p<0.05; ***p<0.001; ****p<0.0001 vs sham), which peaked at 3 DPI (N; *p<0.05 vs 7 DPI). Neutrophils and monocytes induced ROS production at 1 DPI, (M, O, P; *p<0.05 vs sham), which was reduced at 7 DPI (O, P; *p<0.05; ***p<0.001 vs 1 and 3 DPI). Monocytic ROS peaked at 3 DPI (M, P; **p<0.01 vs 1 DPI), and was reduced at 7 DPI (P; ***p<0.001 vs 3 DPI). Data are mean ± SEM (n=4-5 per group). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; by One-Way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For flow cytometry, fluorophore conjugated primary antibodies were purchased as indicated: Brilliant violet-785 conjugated anti-mouse CD45 (BioLegend; Cat# 103149, RRID:AB_2564590), APC-eFluror-780 conjugated anti-mouse CD11b (Thermo Fisher Scientific; Cat# 47-0112-82, RRID:AB_1603193), Brilliant Violet-421 conjugated anti-mouse Ly6G (BioLegend; Cat# 127627, RRID:AB_10897944), Brilliant Violet–605 conjugated anti-mouse Ly6C (BD Biosciences; Cat# 563011, RRID:AB_2737949), Pe-Dazzle-594 conjugated anti-mouse CD3 (BioLegend; Cat# 100245, RRID:AB_2565882), PE-Cynanine5 conjugated anti-mouse CD4 (Thermo Fisher Scientific; Cat# 15-0041-82, RRID:AB_468695), Alexa-Fluor-700 conjugated anti-mouse CD8 (Thermo Fisher Scientific; Cat# 56-0081-82, RRID:AB_494005), CD121a (IL-1RI) Rat anti-mouse, PE, clone:35F5 (BD Biosciences; Cat # 557489, RRID: AB_396727), PerCP-eFluor 710 conjugated anti-mouse IL-1β (Thermo Fisher Scientific; Cat# 46-7114-82, RRID: AB_2573835), Alexa Fluor 647 conjugated anti-mouse NOX2/gp91 phox (Bioss; Cat# bs-3889R-A647-BSS), Dihydrorhodamine 123 (DHR123; Invitrogen; Cat # D23806),Live/Dead Fixable AQUA (Invitrogen; Cat # L34957).

Techniques: Isolation, Staining, Flow Cytometry

GSK2795039 was administered at 2h, 12h, 1d and 2d post CCI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers followed by intracellular staining of NOX2 and IL-1β and analysed by flow cytometry. CCI increased the absolute number of NOX2 + and IL-1β + CD11b + CD45 Int microglia and CD11b + CD45 hi infiltrating cells in vehicle treated CCI mice (C-F; ****p<0.0001 vs sham veh). GSK2795039 treated CCI mice had reduced #NOX2 + #IL-1β + microglia, but levels failed to reach significance (C). GSK2795039 treated CCI mice had significantly reduced #NOX2 + IL-1β + infiltrating cells (E, F; +++ p<0.001 vs CCI veh). Data are mean ± SEM (n=4-6 per group). ****p<0.0001 vs sham veh, +++ p<0.001 vs CCI veh; Two-Way ANOVA with uncorrected Fisher’s LSD.

Journal: bioRxiv

Article Title: The NOX2-ROS-NLRP3 inflammasome axis in traumatic brain injury

doi: 10.1101/2025.05.16.654460

Figure Lengend Snippet: GSK2795039 was administered at 2h, 12h, 1d and 2d post CCI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers followed by intracellular staining of NOX2 and IL-1β and analysed by flow cytometry. CCI increased the absolute number of NOX2 + and IL-1β + CD11b + CD45 Int microglia and CD11b + CD45 hi infiltrating cells in vehicle treated CCI mice (C-F; ****p<0.0001 vs sham veh). GSK2795039 treated CCI mice had reduced #NOX2 + #IL-1β + microglia, but levels failed to reach significance (C). GSK2795039 treated CCI mice had significantly reduced #NOX2 + IL-1β + infiltrating cells (E, F; +++ p<0.001 vs CCI veh). Data are mean ± SEM (n=4-6 per group). ****p<0.0001 vs sham veh, +++ p<0.001 vs CCI veh; Two-Way ANOVA with uncorrected Fisher’s LSD.

Article Snippet: For flow cytometry, fluorophore conjugated primary antibodies were purchased as indicated: Brilliant violet-785 conjugated anti-mouse CD45 (BioLegend; Cat# 103149, RRID:AB_2564590), APC-eFluror-780 conjugated anti-mouse CD11b (Thermo Fisher Scientific; Cat# 47-0112-82, RRID:AB_1603193), Brilliant Violet-421 conjugated anti-mouse Ly6G (BioLegend; Cat# 127627, RRID:AB_10897944), Brilliant Violet–605 conjugated anti-mouse Ly6C (BD Biosciences; Cat# 563011, RRID:AB_2737949), Pe-Dazzle-594 conjugated anti-mouse CD3 (BioLegend; Cat# 100245, RRID:AB_2565882), PE-Cynanine5 conjugated anti-mouse CD4 (Thermo Fisher Scientific; Cat# 15-0041-82, RRID:AB_468695), Alexa-Fluor-700 conjugated anti-mouse CD8 (Thermo Fisher Scientific; Cat# 56-0081-82, RRID:AB_494005), CD121a (IL-1RI) Rat anti-mouse, PE, clone:35F5 (BD Biosciences; Cat # 557489, RRID: AB_396727), PerCP-eFluor 710 conjugated anti-mouse IL-1β (Thermo Fisher Scientific; Cat# 46-7114-82, RRID: AB_2573835), Alexa Fluor 647 conjugated anti-mouse NOX2/gp91 phox (Bioss; Cat# bs-3889R-A647-BSS), Dihydrorhodamine 123 (DHR123; Invitrogen; Cat # D23806),Live/Dead Fixable AQUA (Invitrogen; Cat # L34957).

Techniques: Isolation, Staining, Flow Cytometry

GSK2795039 was administered at 2h, 12h, 1d and 2d post CCI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers, followed by intracellular staining of NOX2 and IL-1β, and analysed by flow cytometry. CCI increased the absolute number of NOX2 + and IL-1β + neutrophils and monocytes in vehicle treated CCI mice (Ai-iv, Bi-iv; ****p<0.001 vs sham veh). GSK2795039 reduced #NOX2 + #IL-1β + neutrophils (Ai-iv; + p<0.05; ++ p<0.01; ++++ p<0.0001 vs CCI veh) and #NOX2 + monocytes (Biii; + p<0.05 vs CCI veh). Data are mean ±SEM (n=4-6 per group). ****p<0.0001 vs sham veh, + p<0.05; ++ p<0.01; ++++ p<0.0001 vs CCI veh; Two-Way ANOVA with uncorrected Fisher’s LSD.

Journal: bioRxiv

Article Title: The NOX2-ROS-NLRP3 inflammasome axis in traumatic brain injury

doi: 10.1101/2025.05.16.654460

Figure Lengend Snippet: GSK2795039 was administered at 2h, 12h, 1d and 2d post CCI. Mononuclear cells isolated from the ipsilateral cortex were stained with surface markers, followed by intracellular staining of NOX2 and IL-1β, and analysed by flow cytometry. CCI increased the absolute number of NOX2 + and IL-1β + neutrophils and monocytes in vehicle treated CCI mice (Ai-iv, Bi-iv; ****p<0.001 vs sham veh). GSK2795039 reduced #NOX2 + #IL-1β + neutrophils (Ai-iv; + p<0.05; ++ p<0.01; ++++ p<0.0001 vs CCI veh) and #NOX2 + monocytes (Biii; + p<0.05 vs CCI veh). Data are mean ±SEM (n=4-6 per group). ****p<0.0001 vs sham veh, + p<0.05; ++ p<0.01; ++++ p<0.0001 vs CCI veh; Two-Way ANOVA with uncorrected Fisher’s LSD.

Article Snippet: For flow cytometry, fluorophore conjugated primary antibodies were purchased as indicated: Brilliant violet-785 conjugated anti-mouse CD45 (BioLegend; Cat# 103149, RRID:AB_2564590), APC-eFluror-780 conjugated anti-mouse CD11b (Thermo Fisher Scientific; Cat# 47-0112-82, RRID:AB_1603193), Brilliant Violet-421 conjugated anti-mouse Ly6G (BioLegend; Cat# 127627, RRID:AB_10897944), Brilliant Violet–605 conjugated anti-mouse Ly6C (BD Biosciences; Cat# 563011, RRID:AB_2737949), Pe-Dazzle-594 conjugated anti-mouse CD3 (BioLegend; Cat# 100245, RRID:AB_2565882), PE-Cynanine5 conjugated anti-mouse CD4 (Thermo Fisher Scientific; Cat# 15-0041-82, RRID:AB_468695), Alexa-Fluor-700 conjugated anti-mouse CD8 (Thermo Fisher Scientific; Cat# 56-0081-82, RRID:AB_494005), CD121a (IL-1RI) Rat anti-mouse, PE, clone:35F5 (BD Biosciences; Cat # 557489, RRID: AB_396727), PerCP-eFluor 710 conjugated anti-mouse IL-1β (Thermo Fisher Scientific; Cat# 46-7114-82, RRID: AB_2573835), Alexa Fluor 647 conjugated anti-mouse NOX2/gp91 phox (Bioss; Cat# bs-3889R-A647-BSS), Dihydrorhodamine 123 (DHR123; Invitrogen; Cat # D23806),Live/Dead Fixable AQUA (Invitrogen; Cat # L34957).

Techniques: Isolation, Staining, Flow Cytometry